Eficácia de soluções desinfetantes na eliminaşão de fungos de importância médica e veterinária

Abstract

Hygiene and disinfection programs are essential in the environmental control of fungal agents potentially pathogenic to humans and animals. In this study it was evaluated the antifungal activity of 4% sodium hypochlorite and 6.6% chlorexidine digluconate solutions against twelve fungal isolates. Fungi such as Aspergillus spp., Candida spp., Microsporum spp., Malassezia pachydermatis, Cryptococcus neoformans and Sporothrix schenckii obtained from environment and clinical cases were tested. Broth microdilution and agar diffusion techniques describes by the Clinical and Laboratory Standard Institute (CLSI) adapted to the chemical solutions were utilized. All isolates were sensible in concentrations minor of the recommended by manufacture of the chlorexidine digluconate (6.6%) with minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) ≤0.42 to 1.68%. To sodium hypochlorite was observed that 58.3% of the isolates were resistant to the concentration recommended by manufacturer (4%) with MIC and MFC values between 2 and >8%. Chlorexidine digluconate presented greater inhibition zone than sodium hypochlorite, with halos of even 31mm of diameter. Chlorexidine demonstrated fungicidal action in low concentrations being efficient in the elimination of mycelial fungi and yeasts of medical and veterinary importance, while the sodium hypochlorite only obtained this action in high concentrations.