Determination of Carnosine, Anserine, and Balenine in the Muscle of Animal

Abstract

The ion-exchange method of Spackmanet al. for determining the basic amino acids as well as carnosine and anserine was modified so that it is applicable to samples including balenine, and the method using a 50 cm column of Arnberlite IR-120 and 0.38 N citrate buffer solution of pH 4.06 at 50°C was recommended. The survey of carnosine, anserine, and balenine on the muscle extracts of various animals (mammals, 23 specimens; domestic fowl, 1; snakes, 3; fishes, 28; crustaceans, 3; molluscs, 6; and sea cucumber, 1) was undertaken in order to obtain unequivocal in- formation about the distribution of these compounds, and it was found that considerable differences in the pattern exist among the species of animals. © 1970, The Japanese Society of Fisheries Science. All rights reserved.