The influence of pore geometry in CP Ti-implants - A cell culture investigation

Abstract

Biocompatibility testing of differently structured titanium implants was performed using an in vitro test system of a newly established human fetal osteoblastic cell line (hFOB 1.19). Cell adhesion of osteoblastic cells on the different porous geometries and the suitability of a copper vapor laser system for surface structuring was tested with the following parameters: cell-number, cell viability, alkaline phosphatase expression. The analysis of the cell culture results demonstrated that 25μm and 200μm porous geometries showed similar or even better results than the negative control of polystyrene; there was no sign of toxic effects. However, the 100μm porous geometry showed an impressive negative influence on the calculated parameters. The reason for this effect is unclear. The series with 50μm, 300μm, 400μm and 500μm showed a comparable, intermediate effect on the cell culture with respect to the different parameters. However, the results were worse than with the 25 and 200μm porous geometry. In conclusion, the 25μm and 200μm porous geometry seems to have the most positive effect on the human osteoblastic cell line hFOB 1.19.