Temperature induced conformational transitions of elastin-like polypentapeptides studied by Raman and NMR spectroscopy

Abstract

Raman and NMR spectroscopy together with DFT quantum chemical calculations have been used to elucidate conformational changes occurring during inverse temperature transition in water solution of two elastin-like polypentapeptides, poly(G1V1G2V2P) and poly(AV1GV2P). From the temperature dependences of Raman spectra and 1H-1H NOESY spectra follows that above the transition temperature a β(II)-turn conformational structure is formed on the residue located between Pro and Val1.