Antioxidant activity from ethanol extract of kupa leaves (Syzygium polycephalum(Miq.) Merr& L. M.Perry) using DPPH (2,2-diphenyl-1-picrylhydrazil) method

Abstract

Kupa is one of the endemic plants from Indonesia belong to guava family (Myrtaceae). This research conducted to determine antioxidant activity of kupa leaves (Syzygiumpolycephalum (Miq.) Merr& L. M. Perry) ethanol extract. Extraction of kupa leaves was carried out by maceration method using ethanol 96%. The antioxidant activity test was done qualitatively and quantitatively. The qualitative antioxidant test was carried out by Thin Layer Chromatography (TLC) using mobile phase chloroform: methanol: formic acid (8: 2: 1) and DPPH 0.2% spotting appearance. Quantitative testing of antioxidant was carried out using DPPH (2,2-diphenyl-1-picrylhydrazil) assays. The TLC test results showed yellow spot on the DPPH with Rf value 0.381. Kupa leaves ethanol extract had the lowest IC50 of DPPH scavenging activity 10.327 ppm which was a very strong antioxidant.