The influence of probiotic on dadiah as fermented buffalo milk product for breastfeeding women with normal bacterial flora of the gastrointestinal tract

Abstract

INTRODUCTION: Dadiah is a fermented buffalo milk product containing many potential lactic acid bacteria as probiotics. Lactic acid bacteria and their derivative products can prevent the onset of various diseases such as spur health and heart work, good food to increase stamina and endurance, prevent colorectal cancer, and improve intestinal microflora. AIM: This study aims to determine the influence of probiotic on dadiah as fermented buffalo milk products for breastfeeding women with normal bacterial flora of the gastrointestinal tract. METHODS: The study was conducted on 28 breastfeeding mothers who were divided into two groups with the same proportion, i.e., mothers who received dadiah ice cream and not. Interventions were performed for 1 month and analysis was performed on an infant’s feces. DNA isolated from feces and Lactobacillus plantarum concentrations were analyzed using real-time polymerase chain reaction. The standard curve designed to get the concentration of bacteria appropriately. Data analysis used SPSS version 20.0. Numerical data were analyzed by used unpaired t-test and paired t-test. A p < 0.05 was considered statistically significant. RESULTS: The results showed that the mean intestinal microflora from the infant before the dadiah ice cream given was 4.2 × 107 CFU/g and after the dadiah ice cream was 8.6 × 108 CFU/g (p < 0.05) while in the control group, the mean L. plantarum intestinal infants before administered whey ice cream is 2.6 × 107 CFU/g and thereafter 1.3 × 107 CFU/g (p > 0.05). CONCLUSION: Based on the results of this study, it can be concluded that there is an increase in L. plantarum concentration after the provision of dadiah ice cream in breastfeeding mothers.