Antibacterial screening of soil bacteria isolates from Guimaras Island, Philippines against escherichia coli and Staphylococcus aureus

Abstract

This study was conducted to screen and determine the antibacterial effects of soil bacterial isolates from three sampling sites of Sinapsapan, Jordan, Guimaras against Staphylococcus aureus and Escherichia coli. Test bacteria were isolated and characterized based on colonial morphology and cell characteristics. Soil bacteria isolates were screened for their antibacterial properties against S. aureus and E. coli using agar disc diffusion method. Each treatment was done in three replicates and trials. There were six bacterial isolates in each sampling site obtained and exhibited antibacterial properties on S. aureus and E. coli after 72 hours of incubation. For S. aureus, I4 (M=35.33 mm, active) from high sampling site; I2 (M=29.44 mm, partially active) from intermediate sampling site, and I1 (M = 38.00 mm, active) from low sampling site while for E. coli, I4 (M= 34.22, active) from high sampling site, I6 (32.78 mm, active) from intermediate sampling site, and I5 (M=34.78, active) from low sampling site had the highest zones of inhibition.