Effect of Storage at Temperature (4C) on Complete Blood Count Parameters

Abstract

Abstract: Background: Complete blood counts are done to monitor overall health, to screen for some diseases, to confirm a diagnosis of some medical conditions, to monitor a medical condition, and to monitor changes in the body caused by medical treatments. Most blood counts today include a CBC count and leukocyte differential count (LDC) (that is, not just the total WBC count but also the count of each WBC type, such as neutrophils, eosinophils, basophils, monocytes, and lymphocytes). More sophisticated modern analyzers can provide extended differential counts. Objective: This study aimed to assess the effect of Storage and Temperature (4C) on Complete Blood Count Parameters. Materials and Methods: This were cross sectional study conducted in Khartoum state in 2016. It included 100 samples, complete blood count conducted to all 100 samples freshly, after 24hours, 48 hours and 72 hours. Mindary BC-2800 hematological analyzer was used to estimate CBC. Results: There was a significant difference in Hematocrit, mean cell volume, MCHC, red cell distribution width, Lymphocytes, granulocyte %, and platelets indices (P less than 0.05). Lymphocytes count, Mixed count, Lymphocytes %, Mixed % and PCT did not show statistically difference ant it were insignificant with P. values (P=0.067), (P=0.080), (P=0.072), (P=0.088) and (P=0.060) respectively. Conclusion: This study concluded that storage caused significant difference in Hematocrit, mean cell volume, MCHC, red cell distribution width, Lymphocytes, granulocyte %, and platelets indices (P less than 0.05)