Effect of Menthe and Green Tea Volatile Oil Extract on Streptococcus mutans, a Bacterium Species Isolated from Dental Caries

Abstract

Objec&ves: To iden(fy phytochemical components, evaluate the yield or vola(le oil extrac(on, and assess the impact of vola(le oil extract on a strain of Streptococcus mutans that was isolated from a dental caries. Materials and Methods: Menthe spicata (menthe) and Camellia sinensis (green tea) were harvested fresh during the flowering period of 2022, and their vola(le oils were extracted for about 24 hours using a sox let apparatus. Green tea and menthe were prepared at four different concentra(ons (50, 75, 100, and 150 mg/ml). Streptococcus mutans growth isolates were inhibited, and the results were compared with extracts from both kinds of plants. Results: Menthe produced the most vola(le oil extract (3%), while green tea produced the least (2%). Alkaloid, phenol, glycoside, and flavonoid showed posi(ve results in the phytochemical screening of M. spicata vola(le oil extract, but saponin showed nega(ve results. The results also showed that the vola(le oil of green tea and menthol oils (100 and 150 mm/ml) were more inhibitory than those of 50 and 75 mm/ml. Overall, given these results, vola(le oil of menthe has greater effect on dental caries compared to vola(le oil of green tea. Conclusions: The vola(le oil extract on menthe inhibitor is more effec(ve on the S. mutans bacteria compared to vola(le oil of the green tea.