Selective action of acetogenin mitochondrial complex I inhibitors

Abstract

Five annonaceous acetogenins, rolliniastatin-1 (1), rolliniastatin-2 (2), laherradurin (3), squamocin (4), annonacin (5), and rotenone as a reference, differing in their NADH oxidase inhibition activity, have been evaluated for antifeedant, insecticidal, trypanocidal and cytotoxic effects on insect, mammalian and tumor cells. All the test compounds were toxic to Leptinotarsa decemlineata, demonstrated selective cytotoxicity to insect Sf9 cells and a panel of tumor cell lines with the multidrug-resistant SW480 (P-glycoprotein+, Pgp+) being the most sensitive one. Compounds 1, 2, 4, and rotenone had post-ingestive effects against Spodoptera littoralis larvae while 1, 4, 5, and rotenone were active against Trypanosoma cruzi. Based on their biochemical properties (inhibition of the mitochondrial NADH oxidase activity), the in vivo effects of these compounds on S. littoralis and their cytotoxic effects on Sf9 and tumor cells were more predictable than their effect on T. cruzi and mammalian cells.