The anti-inflammatory activity of hydrolyzed virgin coconut oil towards RAW 264.7 cell

Abstract

Inflammation can result from the introduction of foreign things into the body, such as bacteria or viruses. Inflammation activates macrophages and mast cells, which serve as immunological agents. The resultant hydrolysis of virgin coconut oil (HVCO) has an anti-inflammatory effect. This research aimed to determine how HVCO affects anti-inflammatory effects in vitro RAW 264.7 cells were activated against lipopolysaccharide. HVCO has anti-inflammatory effects determined by performing a live-cell viability assay using the MTT method [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide], IL-6, TNF-α, IL-1β, iNOS, COX-2, and β-actin gene expression have been studied utilizing reverse transcription-polymerase chain reaction (RT-PCR). The HVCO test results on RAW 264.7 cells with the cell viability test at concentrations (62.5 g/mL; 31.5 g/mL) showed the percentage of live cells (> 90%), namely (97.74 ± 0.31; 102.31 ± 1.21) and assays using the expression of iNOS, TNF-α, IL-6, IL-1β, COX-2, and β-actin genes from HVCO in cells induced with LPS decreased the density value of HVCO, the expression of iNOS and IL-1β resulted in density values the best (0.72±0.010) and (2.40±0.015), TNF-α (0.76±0.7633), IL-6 (1.16±0.010), COX-2 (0.98 ± 0.010), and β-actin (1,02± 0,010). This study showed that HVCO has anti-inflammatory actions on RAW 264.7 cells caused by lipopolysaccharide.