Diagnosis of latent Mycobacterium tuberculosis infection: Tuberculin test versus interferon-gamma release

Abstract

Introduction: The treatment of individuals with active tuberculosis (TB) and the identifi cation and treatment of latent tuberculosis infection (LTBI) contacts are the two most important strategies for the control of TB. The objective of this study was compare the performance of tuberculin skin testing (TST) with QuantiFERON-TB Gold In TUBE® in the diagnosis of LTBI in contacts of patients with active TB. Methods: Cross-sectional analytical study with 60 contacts of patients with active pulmonary TB. A blood sample of each contact was taken for interferon-gamma release assay (IGRA) and subsequently performed the TST. A receiver operating characteristic curve was generated to assess the cutoff points and the sensitivity, predictive values, and accuracy were calculated. The agreement between IGRA and TST results was evaluated by Kappa coeffi cient. Results: Here, 67.9% sensitivity, 84.4% specifi city, 79.1% PPV, 75% NPV, and 76.7% accuracy were observed for the 5mm cutoff point. The prevalence of LTBI determined by TST and IGRA was 40% and 46.7%, respectively. Conclusions: Both QuantiFERON-TB Gold In TUBE® and TST showed good performance in LTBI diagnosis. The creation of specifi c diagnostic methods is necessary for the diagnosis of LTBI with higher sensitivity and specifi city, preferably with low cost and not require a return visit for reading because with early treatment of latent forms can prevent active TB.