Salt Tolerance of Barak Cenana Rice (Oryza sativa L cv. Barak Cenana) EMS Derived M1 Putative Mutants

Abstract

Barak Cenana (Oryza sativa L cv. Barak Cenana) is a native red rice cultivar of Tabanan regency, Bali. We have previously created first-generation putative mutants (M1) lines of Barak Cenana using EMS. In this study, we aim to evaluate M1 lines tolerance to salt stress. Three-leaf seedlings of WT Barak Cenana and M1 lines were cultured in hydroponic system containing Yoshida solution and 100 mM NaCl for 7 days. The salt tolerance level of each seedling was evaluated using SES standard and the survived seedlings were transferred into the pot-containing soil growth for maturation. All WT were died when treated with 100 mM NaCl for 7 days. In contrast, some M1 lines were survived where 0.5% were highly tolerance (II2A-4) and 4.06% (1F-4, 1F-3, II2A-8, IID-1, IID-4, IID-6 and 1B-6) were tolerance to salt stress. This variation of salt tolerance level among M1 lines is likely due to the random mutation caused by EMS. Furthermore, all survived mutants were fertile and able to produce mature seeds. As characters in M1 generation are not stable, future studies are required to establish stable mutant lines. Keywords: Rice, EMS, Mutants, Barak Cenana, salt tolerance