Biochemical determination of lipid content in hepatic steatosis by the Soxtec method

Abstract

AIM: To establish a quantitative method to measure the amount of lipids. METHODS: The livers of 53 male Wistar rats (225 g) with different degrees of hepatic steatosis were studied. This model of hepatic steatosis was based on a high carbohydrate, fat-free modified diet. Biopsies were classified into four grades depending on fat accumulation, using the Kleiner and Brunt classification. Total fat was studied by the Soxtec method (Soxtec™ 2050 Auto Fat Extraction System), and agreement between both assays was assessed by calculating the κ coefficient. RESULTS: According to the histological classification, 38% of rats presented grade 0, 21% grade 1, 22% grade 2 and 20% grade 3. The amount of fat per 100 g tissue was 2.60 ± 0.64 g for grade 0, 3.87 ± 1.59 g for grade 1, 5.82 ± 1.37 g for grade 2 and 8.68 ± 2.30 g for grade 3. Statistically significant differences were found between the mean values for each of the histological grades (P < 0.05). The correlation for the quantification of fat in the liver between both assays was moderate (κ = 0.60). CONCLUSION: The biochemical quantification of fat in liver tissue by the Soxtec method was correlated with the histological classification, although the agreement between the two tests was only moderate. © 2010 Baishideng.