Synthesis, characterization, and in Vitro antimicrobial evaluation of hydrazone and bishydrazone derivatives of isatin

Abstract

A new series of hydrazone and bishydrazone derivatives was synthesized starting from adipic dihydrazide, oxalyl dihydrazide, 4-hydroxybenzhydrazide, 2-furancarboxylic acid hydrazide, acetohydrazide, 4-pyridinecarboxylic acid hydrazide, and isatin. The chemical structures were confirmed by means of 1H-NMR, UV, and IR spectral data and elemental analysis. The synthesized compounds were evaluated in vitro as antimicrobial agents against representative strains of Gram-positive (clinical strains of Staphylococcus saprophyticus, Bacillus cereus, Enterococcus faesium), Gram-negative bacteria (clinical strains of Klebsiella pneumonia, Escherichia coli, Serratia marcescens, Pseudomonas aeruginosa, Shigella dysenteriae, Salmonella typhi), and antifungal agent against Candida albicans (clinical isolated) by the minimal inhibitory concentration (MIC) method. MIC was defined as the lowest concentration of compound inhibiting the growth of each strain. All the bacteria and fungi studied were screened against some antibiotics and their chemical zone diameters compared. © 2010 Springer Science+Business Media, Inc.