Bacteriophage Ps1.JH, Effectively Controls Pseudomonas aeruginosa of Diabetic Foot Ulcer Origin

Abstract

Introduction: Pseudomonas aeruginosa is an important etiological agent of diabetic foot ulcers. It is included in the World Health Organization’s priority list of organisms for which new antibiotics or alternatives are urgently needed. The rise in antimicrobial resistance highlights the urgent need to explore alternatives to antimicrobial drugs. Methods: In this study, we isolated the bacteriophage Ps1.JH from hospital sewage water targeting a locally isolated strain of Pseudomonas aeruginosa. Results: Ps1.JH can inhibit bacterial growth for up to 24 h. It is stable at pH 4–9 and temperature up to 60°C. Ps1.JH has double stranded DNA genome of 72.67 kb with 55% GC content, encoding 91 open reading frames. According to ICTV classification, Ps1.JH belongs to the genus Litunavirus of Schitoviridae family. It does not encode any integrase, repressor, virulence factor, or toxin. Conclusion: Ps1.JH could be a potential candidate for controlling P. aeruginosa of diabetic foot ulcer origin after further analyses.