Differential actions of urocortins on neurons of the myenteric division of the enteric nervous system in guinea pig distal colon

Abstract

Background and purpose: Urocortins (Ucns) 1, 2 and 3 are corticotropin-releasing factor (CRF)-related neuropeptides and may be involved in neural regulation of colonic motor functions. Nevertheless, details of the neural mechanism of action for Ucns have been unclear. We have, here, tested the hypothesis that Ucns act in the enteric nervous system (ENS) to influence colonic motor behaviour. Experimental approach: We used intracellular recording with 'sharp' microelectrodes, followed by intraneuronal injection of biocytin, and immunohistochemical localization of CRF 1 and CRF 2 receptors in guinea pig colonic tissue. Key results: Application of Ucn1 depolarized membrane potentials and elevated excitability in 58% of AH-type and 60% of S-type colonic myenteric neurons. In most of the neurons tested, depolarizing responses evoked by Ucn-1 were suppressed by the CRF 1 receptor antagonist NBI 27914, but were unaffected by the CRF 2 receptor antagonist antisauvagine-30. The selective CRF 2 receptor agonists, Ucn2 and Ucn3, evoked depolarizing responses in 12 and 8% of the AH-type myenteric neurons, respectively, and had no effect on S-type neurons. Antisauvagine-30, but not NBI 27914, suppressed these Ucn2- and Ucn3-evoked responses. Immunohistochemical staining identified CRF 1 as the predominant CRF receptor subtype expressed by ganglion cell somas, while CRF 2-immunoreactive neuronal somas were sparse. Ucns did not affect excitatory synaptic transmission in the ENS. Conclusions and implications: The results suggest that Ucns act as neuromodulators to influence myenteric neuronal excitability. The excitatory action of Ucn1 in myenteric neurons was primarily at CRF 1 receptors, and the excitatory action of Ucn2 and Ucn3 was at CRF 2 receptors. © 2009 The British Pharmacological Society.