The phytochemical analysis and antimicrobial activity of pergularia to mentosa in North East Kingdome of Saudi Arabia KSA

Abstract

The increment in numbers of antimicrobial resistant strains along with the scarcityof new targets for drugindustryhas forcedscientists to investigate deeply in the natural resources for new compounds with antimicrobial activity. Pergularia tomentosa is a member of the Apocynaceae family found in a wide geographical region including the Gulf region, Africa,andthe Middle East. It is used as a remedy for the treatment of skin sores, asthma,and bronchitis. Dried plants of Pergularia tomentosa were subjected to extraction by using a Soxhlet extractor process to obtain essential oil and characterized by HPLC- Mass Spectroscopy (GC-MS). The essential oil was evaluated forantibacterial activity against pathogenic microorganisms by well diffusion method and confirmed by microdilution method. Additionally, we measured the antioxidant activity of the extracts using DPPH reagent. Phytochemical analysis has revealed variation in compositions and concentrations of P. tomentosa constituents grown in Hail from otheragricultural regions. The lowest MIC was recorded withethylacetate extract MIC of 6.25 mg/ml against S. typhi, whereas, the ethanolic extract had the broadest effective against the five strains with a MIC of 25 mg/ml. In conclusion, we summarize a variation between the phytochemical constituents of P. tomentosaplants grown in the district of Hail and other geographical regions. In addition, there are several natural phytocompounds with an antimicrobial activity could be a good target for theantimicrobial and antioxidants industry.