Use of C18 reversed-phase liquid chromatography for the isolation of monoterpene glycosides and nor-isoprenoid precursors from grape juice and wines

Abstract

Glycosidic derivatives of monoterpene flavourants of grapes and wines can be preparatively isolated by selective retention on a C18-bonded reversed-phase adsorbent. These components can thus be concentrated by a factor of 20,000 in a single chromatographic step. Class separation of monoterpene glycosides at different oxidation levels can also be achieved on the reversed-phase adsorbent. Teh process has also led to the discovery of precursors of 2-phenylethanol, benzyl alcohol, damascenone, vitispirane and 1,1,6-trimethyl-1,2-dihydronaphthalene. Additionally, many other related nor-isoprenoid compounds not previously known in grapes have been observed. © 1982.