Determination of antioxidant compounds from leaf and stem of Angelica keiskei by gas chromatography-mass spectrometry

Abstract

In this paper, the antioxidant activities of leaf extract and stem extract from Angelica keiskei were investigated employing 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Leaf extract showed significant radical scavenging activity which was close to that of rutin, an excellent antioxidant compound while the radical scavenging activity of stem extract was a little weak. Subsequently, gas chromatography-mass spectrometry (GC-MS) was employed for determination of antioxidant compounds of the leaf extract and stem extract. Results indicated that 3,5-dihydroxy-6-methyl-2H-pyran-4(3H)-one was the main antioxidant compound in leaf extract and stem extract, fatty acid/ester and unsaturated fatty acid/ester are other kind of antioxidant compounds present in leaf extract.