MP51-05 URINARY MICROBIOTA IN PATIENTS WITH UROLOGIC MALIGNANCIES

Abstract

INTRODUCTION AND OBJECTIVE: Dysbiosis of the urinary microbiome has also been identified as the cause of various urological disorders, such as urgency urinary incontinence, overactive bladder and prostate cancer. The aim of this study was to characterize and compare the urinary microbiome of prostate cancer patients with those of healthy controls. METHODS: The study was conducted with urinary DNA of 54 cancer patients and 17 healthy individuals. We performed a 16S rRNA gene sequencing using Illumina MiSeq to analyze the urine microbiota. Assignment of taxonomy was performed on using pipeline of QIIME taxonomy classification methods. Generalized linear models implemented in multivariate association with linear models (MaAsLin) packages of RStudio were used for quantitative and qualitative analysis. RESULTS: Of the 71 samples, we excluded samples (8 samples of the cancer group and 9 samples of the healthy control group) that showed too low sequencing reads (less than 3500 reads). A total of 54 samples were analyzed, including 46 from cancer group (21 bladder cancer, 16 prostate cancer, and 9 renal cell cancer) and 8 healthy controls. There was no significant difference in age between cancer and healthy control group. Prostate cancer groups (n=16) had a distinct bacterial community compared to healthy control group (n=8) and other cancer groups (n=30) (Figure 1). Also, some bacterial genera (e.g., Peptoniphilus, Pseudomonas, Propionibacterium q < 0.25) were significantly enriched in prostate cancer groups compared to healthy control group and other cancer groups (Figure 2). CONCLUSIONS: The overall urinary micribiome composition of prostate cancer patients was different from other urologic malignancies and healthy control group. (Figure Presented).