Micropropagation of Vanilla (Vanilla planifolia Andrews) with Modification of Cytokinins

Abstract

The constraints of vanilla development in conventional vegetative propagation is the limitations of the parent plant as stem cuttings. The ability of the explants to regenerate and differentiate to form buds in vitro is excessive need to be controlled through cytokinin regulation to multiplication of vanilla seedlings as a parent, healthy and uniform. The objectives of this research is the effect of BAP and Kinetin to shoot multiplication vanilla. The study was conducted in the Tissue-Culture Politeknik Negeri Jember using a Completely Randomized Design (CRD). Treatment in the form of the addition of cytokinins in MS basal medium. Factor 1 is BAP concentration 0 mg.L-1, 1 mg.L-1, 2 mg.L-1 and 3 mg.L-1. Factor 2 is Kinetin concentration 0 mg.L-1, 1 mg.L-1 and 2 mg.L-1. Data analysis used the DMRT test of 5%. The result showed the emergence of vanilla shoots is not influenced by exogenous growth regulators and addition of BAP 3 mg.L-1 gave the most multiplication results of 3-4 shoots with shoot lengths of 2-2.5 cm at 28 days after inoculation.