Novel bioactive flavonoid 5-hydroxy-2-(8-hydroxy 4-oxo-4H-chromen-2-yl)-4-oxo-3,4-dihydro-2H-1-benzopyran-7-yl-N-[(2-methylpropoxy) carbonyl] carbamate from Kalanchoe pinnata

Abstract

Kalanchoe pinnata is a conspicuous medicinal plant and finds wide applications in pharmaceutical industries. Phytochemical studies have established the mode of action of plant extract as bioactive agent. The foremost constituents accountable for biological activity are polyphenols, which are an important class of naturally occurring antioxidants, having innumerable biological activities such as anticancer, antifungal, antibacterial, antiviral and anti-cholesterol. The present study is focussed on extraction, identification and characterization of flavonoids present in leaves of Kalanchoe pinnata. The purified fractions of the methanol extract have been quantised by preparative TLC and RP-HPLC to achieve maximum purity. The isolated purified compounds have been studied for spectral characterisation like IR, 1H and 13C NMR and MS. On the basis of chemical constitution and spectral analysis, the structure has been elucidated as a 5-hydroxy-2-(8-hydroxy 4-oxo-4H-chromen-2-yl)-4-oxo-3, 4-dihydro-2H-1-benzopyran-7-yl N-[(2-methylpropoxy) carbonyl] carbamate. Combined bioactivities are yet to be evaluated along with other known compounds like gallic acid and quercetin.