Lamin A/C is expressed in pluripotent mouse embryonic stem cells

Abstract

The pluripotent nature of embryonic stem cells (ESC) is associated with a dynamic open chromatin state and an irregular nuclear shape. It has been postulated that the absence of Lamin A/C contributes to these features. However, we show that mouse ESC s express low, yet readily detectable, amounts of Lamin A/C at both the RNA and protein levels. Fulllength transcripts of both isoforms were readily detected by q-PC R and deep RNA sequencing. Additionally, protein expression was validated in multiple primary and established ESC lines by immunoblotting using several independent antibodies. Immunofluorescence labeling showed localization of Lamin A/C at the nuclear periphery of all Oct4/Nanog double-positive ESC lines examined, as well as in the inner cell mass of blastocysts. Our results demonstrate ESC s do express low levels of Lamin A/C, thus models linking pluripotency and chromatin dynamics with the absence of Lamin A/C need to be revisited. © 2013Landes Bioscience.