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Protocol for the Electroporation of Neurospora Spheroplasts

  • Kothe G
  • Free S
Fungal Genetics Reports (1996) 43(1) 31-32
DOI: 10.4148/1941-4765.1309
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Abstract

A protocol for electroporating Neurospora strains is described. This protocol uses spheroplasts for transformation rather than germinated conidia with enzyme-weakened walls. High efficiency transformation is obtained with this system even with batches of Novozyme234 which worked poorly in the PEG based protocol.

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Kothe, G. O., & Free, S. J. (1996). Protocol for the Electroporation of Neurospora Spheroplasts. Fungal Genetics Reports, 43(1), 31–32. https://doi.org/10.4148/1941-4765.1309

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