The genomic DNA sequence of a novel enteric uncultured microphage, φφCA82 from a turkey gastrointestinal system was determined utilizing metagenomics techniques. The entire circular, single-stranded nucleotide sequence of the genome was 5,514 nucleotides. The φCA82 genome is quite different from other microviruses as indicated by comparisons of nucleotide similarity, predicted protein similarity, and functional classifications. Only three genes showed significant similarity to microviral proteins as determined by local alignments using BLAST analysis. ORF1 encoded a predicted phage F capsid protein that was phylogenetically most similar to the Microviridae MH2K member's major coat protein. The φCA82 genome also encoded a predicted minor capsid protein (ORF2) and putative replication initiation protein (ORF3) most similar to the microviral bacteriophage SpV4. The distant evolutionary relationship of φCA82 suggests that the divergence of this novel turkey microvirus from other microviruses may reflect unique evolutionary pressures encountered within the turkey gastrointestinal system. © 2011 Zsak et al; licensee BioMed Central Ltd.
CITATION STYLE
Zsak, L., Day, J. M., Oakley, B. B., & Seal, B. S. (2011). The complete genome sequence and genetic analysis of φφCA82 a novel uncultured microphage from the turkey gastrointestinal system. Virology Journal, 8. https://doi.org/10.1186/1743-422X-8-331
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