The nucleotide sequence of a wheat γ-gliadin genomic clone

Abstract

γ-Gliadins are a subclass of the storage proteins from wheat. We have isolated from a wheat genomic library a clone encoding a γ-gliadin gene. The amino acid sequence derived from the gene sequence reveals a mature protein (308 amino acids, excluding a 19 amino acid signal sequence) with a characteristic repeated structure of the consensus heptapeptide PQQPFPQ corresponding to the high glutamine (35.4%) and proline (17.5%) content. The gene does not contain an intron, and possesses a typical eukaryotic promoter. © 1986.