Aspek Klinis dan Pemeriksaan Laboratorium untuk Diagnosis Demam Berdarah Dengue

Abstract

Dengue infection is a viral infection through arthropods that is found throughout the world. This disease is caused by dengue virus which consists of four serotypes and each of these serotypes can cause disease. The pathogenesis of dengue infection, especially the mechanism of dengue hemorrhagic fever (DHF) manifestations is still not clearly understood. The results of laboratory tests on dengue hemorrhagic fever found thrombocytopenia (≤ 100,000/mm3), a 20% increase in hematocrit and a positive Rumple Leed test. Five basic serological tests used to detect antibodies in diagnosing dengue infection are haemagglutination-inhibition (HI), complement fixation (CF), neutralization test (NT), IgM capture enzyme-linked immunosorbent assay (MAC-ELISA), and indirect IgG. ELISA. RT-PCR examination in a number of studies was reported to have successfully detected the dengue virus. This examination has better specificity and sensitivity compared to virus isolation with a fast turn around timeLaboratory tests on dengue infection include hematological, serological and nucleic acid tests that play a role in supporting the rapid and accurate diagnosis of dengue hemorrhagic fever.