Oral mucositis (OM) is a painful inflammatory oral condition that affects children who undergo chemotherapy. Oxidative stress is a known OM mediator and pro-inflammatory cytokines contribute to the amplification of the immune response. Objective: To investigate the possible associations of rs4880 (superoxide dismutase 2, SOD2 47 C/T), rs7943316 (catalase, CAT-21 A/T), rs1800629 (tumor necrosis factor α, TNF-α-308 G/A), and rs1800795 (interleukin 6, IL-6-174 G/C) polymorphisms with chemoinduced OM occurrence and severity in oncopediatric patients. Methodology: We conducted a single-center, observational cross-sectional study with sample collection of oral epithelial cells from 95 children and adolescents with hematological cancers who underwent chemotherapy, followed by genomic DNA extraction. Single-nucleotide polymorphisms (SNPs) were assessed with PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). Demographic data and information concerning OM occurrence were obtained from dental charts of the multidisciplinary oral care team. Information on OM severity was obtained from appropriately-filled Oral Assessment Guide records. Descriptive and inferential statistics were conducted with Student’s T test, chi-squared test, and Fisher’s exact test, with p≤0.05. Results: The mean age was 10 years-old and most patients were male individuals (57.89%). Female sex was considered a protective factor for OM occurrence (OR=4.83; CI=[1.14; 16.57]). The AA genotype for CAT was the most frequent amongst individuals with severe OM (p=0.04). The GA genotype for TNF-α was the most frequent amongst individuals without severe OM (p=0.03). For SOD2 and IL-6, the most frequent genotypes were CT and GG respectively for all groups (p>0.05). Conclusion: The AA genotype for CAT-21 A/T was a tendency among the group with severe OM. Data on TNF-α-308 G/A were inconclusive. No associations were detected for SOD2 47 C/T and IL-6-174 G/C polymorphisms in oncopediatric patients with chemo-induced oral mucositis.
CITATION STYLE
Coêlho, M. C., Viana Filho, J. M. C., de Souza, B. F., Valença, A. M. G., Persuhn, D. C., & de Oliveira, N. F. P. (2022). Genetic polymorphisms of genes involved in oxidative stress and inflammatory management in oncopediatric patients with chemoinduced oral mucositis. Journal of Applied Oral Science, 30. https://doi.org/10.1590/1678-7757-2021-0490
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