Dynamin2, clathrin,andlipid rafts mediate endocytosis of the apical Na/K/2Cl cotransporter NKCC2 in thick ascending limbs

Abstract

Background: Apical NKCC2 mediates NaCl reabsorption by the thick ascending limb. Results: Inhibition of dynamin-2, clathrin and lipid raft-mediated endocytosis blunted NKCC2 endocytosis, increasing steadystate surface NKCC2 levels. Conclusion: NKCC2 undergoes constitutive endocytosis in native thick ascending limbs via dynamin-2 and two endocytic pathways. Significance: Endocytic pathways regulate steady-state surface NKCC2 in native thick ascending limbs. Impaired NKCC2 endocytosis may induce salt-sensitive hypertension. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.