Modification of the filter-inserted tip method for simple and rapid DNA extraction from strawberry

Abstract

In order to perform conveniently DNA extraction from strawberry leaves, we tried to apply the “filter-inserted tip method”. This method has been used to extract easily DNA from rice. To examine the utility of the method in strawberry leaves, DNA extraction experiment was carried out, and the yielded DNA samples were used for PCR reactions. PCR reaction was greatly improved with the crushing buffer when 50 mg/ml polyvinylpolypyrrolidone was added. PCR amplicons were obtained for 132–140 out of 144 samples examined, showing high reproducibility of the method. It took approximately 70 min to conduct DNA extractions from 24 samples. It was about 40% of the operation time in the case of using a commercially available DNA extraction kit. The results suggest that this method is suitable for rapid DNA extractions from a large number of samples for PCR reactions, such as marker-assisted selection with simple manipulations, low cost, and without using expensive equipment.