Cytotoxic Effects of Rapana venosa Hemocyanin on Bladder Cancer Permanent Cell Lines

Abstract

The cytotoxic activities of hemocyanin from Rapana venosa and its structural subunits with different oligosaccharide structures are studied in vitro on the bladder cancer permanent cell lines CAL-29, T-24, and the normal urothelial cell line HL 10/29. A significant cytotoxic effect against these tumor cells was observed for the functional unit RvH1-c after a treatment in contrast to native RvH and its structural subunits. Cells, treated with RvH1-c, show a mixture of both, necrotic and apoptotic cells. Cytotoxicity of RvH1-c is probably due to specific oligosaccharide structures exposed on the surface of the glycoprotein.