A type II restriction endonuclease with an eight nucleotide specificity from Streptomyces fimbriatus

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Abstract

A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus. This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN/NGGCC, symmetrically to produce a three base, 3′ extension. © 1984 IRL Press Limited.

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Qiang, B. qin, & Schildkraut, I. (1984). A type II restriction endonuclease with an eight nucleotide specificity from Streptomyces fimbriatus. Nucleic Acids Research, 12(11), 4507–4516. https://doi.org/10.1093/nar/12.11.4507

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