Therapeutic Efficiency of Aristolochic acid on Oral cancer Induced Experimental Rats

Abstract

Aristolochia indica is one of the important medicinal plant sources of bioactive compound aristolochic acid (AA). The compound was found to produce cytotoxicity at higher doses, thus AA was examined for therapeutic efficacy against 4-nitroquinoline 1-oxide (4-NQO) induced oral cancer. Purity of the isolated AA was assayed using UV, IR, NMR, Mass spectral studies were revealed that the maximum wavelength (254 nm), functional groups and nature (NO 2 nitro groups and aromatic), the structure (C 17 H 11 NO 7) and molecular weight (341.28) of the compound AA. The dose of AA at 10 mg/kg b.wt/ for 4 weeks. Effect of AA on oral cancer was studied by exploiting various biochemical and histological parameters such as Glutathione-S-transferase (GST), Gamma gultamyl transferase (GGT), 5'-Nucleotidase (5'-NT) and Cathepsin D (CD) and glycoconjugates. There was significant increase in GST and significant decrease in other markers and glycoconjugates were observed on AA treatment in oral cancer induced rats. Number of tumors, percentage of tumors and tumor volume was found to be decreased on AA treated rats on oral cancer induced rats. Thus AA was found to modulate therapeutically on oral cancer regression.