Resistance Escherichia coli isolates to antibiotics from the organ samples originating from swine farms

Abstract

Bacteria Escherichia coli (E.coli) is a ubiquitous microorganism with a different serotypes, that cause septicemia in neonatal piglets, diarrhea in suckling piglets, diarrhea and edemic disease in weaned piglets. Vaccination and antibiotic treatment are less effective ways in solving the problem, especially when the category of pig is not taken into consideration, as well as feed safety and chemical composition of feed. The resistance of pathogens depends on the farm size, production type and pigs category raised on the farm. In addition, the antibiotics used for veterinary purposes reduce their effectiveness in human medicine. Analyzing the antibiograms results obtained from the isolates of dead piglets with E. coli infection in last two years (2007-2009), it can be concluded that E. coli strains developed resistance to most chemotherapeutics. Applying bacteriological examination it was detected that the isolates of E. coli are highly sensitive to the following chemotherapeutics: Floron (86.36%), Cobactam (71%), Enrofloxacin (54.84%), Neomycin (38.71%), Gentamicin (38.71%), Lincospectin (29%), Flumequine (29%), Amoxicillin (26.66%), Colistin (17.39%), Penicillin (9.67%), Tiamulin (6.45%), Tetracycline (6.45%), Doxycycline (6.45%), Streptomycin (3.22%). Intermediate degree of sensitivity was found with: Flumequin (38.71%), Colistin (34.78%), Cobactam (30%), Tilozin (29.17%), Gentamicin (25%). E. coli strains were the least resistant to Cobactam (not detected), Floron (4.54%), Neomycin (12.9%), Colistin (13.04%), Flumequin (19.35%).Rezistencija Escherichia coli je vezana za velicinu farme, tip proizvodnje i kategoriju svinja na farmi. Pored toga, upotreba antibiotika u veterinarskoj medicini limitira njihovu efikasnost u humanoj medicini. Analizom rezultata antibiograma dobijenih na izolatima u protekle dve godine (period 2007-2009) iz organa uginule prasadi sa znacima koli-infekcije, utvrdjen je razvoj rezistencije sojeva E. coli na veliki broj hemoterapeutika. Bakterioloskim ispitivanjem je ustanovljeno da su izolati E. coli veoma osetljivi na sledece hemoterapeutike: floron, kobaktam, enrofloksacin, neomicin, gentamicin, linkospektin, flumekvin, amoksicilin, kolistin, penicilin, tiamulin, tetraciklin, doksicilin, streptomicin. Srednji stepen osetljivosti uzrocnika je ustanovljen na: flumekvin, kolistin, kobaktam, tilozin, gentamicin. Najmanje rezistentnih sojeva E. coli je bilo na kobactam (nije ustanovljena), floron, neomicin, kolistin, flumekvin. PR Projekat Ministarstva nauke Republike Srbije, br. TR 031071