Biofilm and the role of the ica operon and aap in Staphylococcus epidermidis isolates causing neurosurgical meningitis

Abstract

Fifty-five Staphylococcus epidermidis isolates, classified as contaminants or causing device-related meningitis, from external ventricular drain (EVD) and non-EVD cerebrospinal fluid specimens were characterized. Thirty-three of 42 (78.6%) meningitis isolates were PCR-positive for ica and aap, known determinants of polysaccharide- and protein-mediated biofilm production, whereas five of 13 (38.5%) contaminants were ica - and aap-negative; 71.4% of meningitis isolates and 84.6% of contaminants produced biofilm. ica + aap + meningitis isolates produced more biofilm than ica + aap- isolates (p0.0020). ica+ aap- isolates did not produce more biofilm than ica-aap+ isolates (p 0.4368). Apparently, ica and aap are associated with biofilm production in S.epidermidis device-related meningitis isolates. © 2008 European Society of Clinical Microbiology and Infectious Diseases.