Cytotoxic and antibacterial activities of secondary metabolites from endophytic fungus Pestalotiopsis virgatula VN2

Abstract

A 2014-Cytotoxic and antibacterial activities of secondary metabolites from endophytic fungus Pestalotiopsis virgatula VN2. Abstract Development of new drugs, especially in area of oncology and infectious diseases, represents today one of the most important research. Fungal endophytes are receiving increasing attention by natural product chemists due to their diverse and structurally unprecedented compounds making them interesting candidates for drug discovery. In the present investigation, aims to study the cytotoxic and antibacterial activity of secondary metabolites from enfophytic fungus Pestalotiopsis virgatula VN2. The ethyl acetate extract of partially purified fraction E had strong cytotoxic activity against MCF-7 and MDA MB-231 cell lines with concentration increase to 30 μg/mL the cell viability was decreased by 59.0% and 45.6% respectively. Further, fraction E had an effective antibacterial activity against multidrug resistant S. aureus. The fraction E produced a maximum inhibition zone of 19.8 mm against S. aureus strain 4. The minimal inhibitory concentration of the fraction E was found to be 31.2 to 500 µg/mL against S. aureus strains. In GC-MS analysis, one predominate peak with retention time of 20.9 and molecular ion peak at m/z 278.7 was observed.