Phosphorylation of guanosine using guanosine-inosine kinase from exiguobacterium acetylicum coupled with atp regeneration

Abstract

Guanosine 5′-monophosphate (5′-GMP) and inosine 5′-monophosphate (5′-IMP) are widely used as flavor enhancers. Recently, a novel process for 5′-IMP production by phosphorylation of inosine using guanosine-inosine kinase coupled with ATP regeneration was reported.1) In this study, we demonstrated the practical possibility of producing 5′-GMP by phosphorylation of guanosine using a guanosine-inosine kinase from Exiguobacterium acetylicum coupled with ATP regeneration. © 2000, Taylor & Francis Group, LLC. All rights reserved.