Lipid profiling of cancerous and benign gallbladder tissues by 1H NMR spectroscopy

Abstract

Qualitative and quantitative 1H NMR analysis of lipid extracts of gallbladder tissue in chronic cholecystitis (CC, benign) (n=14), xanthogranulomatous cholecystitis (XGC, intermediate) (n=9) and gallbladder cancer (GBC, malignant) (n=8) was carried out to understand the mechanisms involved in the transformation of benign gallbladder tissue to intermediate and malignant tissue. The results revealed alterations in various tissue lipid components in gallbladder in CC, XGC and GBC. The difference in the nature of lipid components in benign and malignant disease may aid in the identification of the biological pathways involved in the etiopathogenesis of GBC. This is the first study on lipid profiling of gallbladder tissue by 1H NMR spectroscopy, and has possible implications for the development of future diagnostic approaches. © 2010 John Wiley & Sons, Ltd.