The role of genes influencing the corolla in pollination of Antirrhinum majus

Abstract

Studies of pollination ecology have been hindered by an absence of biochemical information about the basis of polymorphism. Using model plants and mutant lines described by molecular genetics may circumvent this difficulty. Mutation of genes controlling petal colour and petal epidermal cell shape in Antirrhinum majus was previously shown to influence fruit set. White flowers set less fruit than magenta flowers and mutants with flat petal epidermal cells set less fruit than flowers with conical cells. Here we analyse the causal pathway underlying this phenomenon through a study of floral characteristics and bee behaviour. Results indicate that bees recognized plants with magenta conical-celled flowers at a distance and did not approach white flowers or magenta flat-celled flowers so frequently. Petal cell shape interacted with colour in determining whether an approaching bee landed on a flower within a plot and whether a bee landing on a flower would probe it. The intrafloral temperature of flowers with conical petal cells was shown to increase with solar irradiance, unlike the intrafloral temperature of flowers with fiat petal cells. The difference in fruit set may reflect pollinator discrimination between genotypes as a consequence of the effect of intrafloral temperature on nectar quality and quantity.