The yeast kinetochore protein Slk19 is required to prevent aberrant chromosome segregation in meiosis and mitosis

Abstract

Background: Slk19 is a coiled-coil protein, which locates to the kinetochores of S. cerevisiae. Most cells lacking Slk19 undergo incomplete meiosis and form dyads during sporulation. Endogenous chromosomes appeared to be predominantly divided in an equational manner during single-division meiosis of slk19 null mutants. Results: We have monitored the segregation of artificial chromosomes (YACs) in slk19 null mutants during both single-division meiosis and complete meiosis. In contrast to the results obtained with endogenous chromosomes, YACs only rarely undergo equational segregation during single division meiosis, although high rates of aberrant segregation were detected. This accounts for the high frequency of lethal spores among dyads of slk19Δ null mutants. The fraction of slk19Δ cells that were able to form tetrads solely exhibited YAC segregation defects in meiosis II, whereas the segregation of YACs in meiosis I was normal in these cells. This result might indicate that correct chromosome division in meiosis I is a pre-requisite for tetrad formation. slk19 null mutants also showed YAC instability in mitosis and reduced survival after the induction of mitotic spindle damage. Conclusion: Slk19 is required to avoid aberrant segregation of chromosomes in meiosis I and II and in mitosis. We suggest that the absence of Slk19 leads to uncoupling of chromosome movement from completion of microtubule attachment and resolution of chromosome cohesion.