Renal histopathology after mixed liquor consumption in Wistar rat

Abstract

In Indonesia illegal traditional liquor mostly contain ethanol and methanol some of illegal liquor Indonesia called cukrik (mixed liquor), Misuse of mixed liquor can lead to intoxication and may lead to death. Several studies have shown its effect on kidney damage. This study is to analyzing the effect of mixed liquor (ethanol and methanol) on the histopathological damage of male Wistar rat kidney. This was experimental laboratories using a post-test only control group design. We are using 28 wistar rats that were divided into four groups, each group consisting of 7 rats. Group control (C) was given 4 mL of distilled water; groups P1, P2, and P3 were given 1ml, 2 ml, and 4 ml cukrik in orally for 14 days. Mixed alcohol contains 4% methanol and 20% ethanol. Examination of histopathological features was carried out by identifying glomerular cells, tubular cells, and interstitial with HE (Hematoxylin and Eosin) stainning using EGTI (Endothelial, Glomerular, Tubular, and Interstitial) scoring. Data were analyze using kruskal wallis and Mann whitney test. With confidence level p<0,01. glomerular and tubular examination there were significant differences with p<0,01, in interstitial examination there was no significant damage found on the instrestitial with p<0,01. Consumption of cukrik produced glomerular and tubular damage on the histopathological image of male Wistar rats' kidneys, but there was no visible damage to the interstitials cell. With higher consumption of mixed liquor will make tubular and glomerular damage heavier.