Indirect Spectrophotometric Estimation of Ciprofloxacin Hydrochloride in Pharmaceuticals using N-Bromosuccinimide and Methylene Blue Dye

Abstract

The present study aims to develop a swift, simple, accurate and sensitive spectrophotometric method for determination of Ciprofloxacin Hydrochloride ( CPFX ) in pure and pharmaceutical formulations. The suggested method depend on oxidation of CPFX with known excess amount of N-bromosuccinimide ( NBS ) in acidic medium and after reaction is insured to be complete, the surplus NBS is estimated by de colorization of methylene blue dye and measuring the absorbance of surplus dye at 665 nm . A linear calibration curve was obtained over the concentration range 0.5 - 4.5 μg.ml-1 with correlation coefficient of 0.9993 . The molar absorptivity and sandell's sensitivity index values were determined to be 8.6 ×104 L.mol-1.cm-1 and 0.004276 μg.cm-2, respectively .The limit of detection (LOD) and quantification (LOQ) were calculated to be 0.0033 and 0.0111 μg.ml-1, respectively . The proposed method has been successfully applied to the determination of CIPH in available dosage form, the validity proposed method was confirmed by recovery study via standard addition technique.