To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40. fmol to 1. pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented. © 2013 Elsevier B.V.
CITATION STYLE
Ahmad-Tajudin, A., Adler, B., Ekström, S., Marko-Varga, G., Malm, J., Lilja, H., & Laurell, T. (2014). MALDI-target integrated platform for affinity-captured protein digestion. Analytica Chimica Acta, 807, 1–8. https://doi.org/10.1016/j.aca.2013.08.051
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