MORPHOLOGICAL, BIOCHEMICAL AND MOLECULAR IDENTIFICATION OF 25 RHIZOBIUM LEGUMINOSARUM ISOLATES FROM FABA BEAN IN MENOFIA GOVERNORATE, EGYPT

Abstract

We collected root nodules of faba bean plants from nine different area in Menofia Governorate. Twenty five isolates were collected from faba bean as a host trap. We identified our tested isolates for morphological characteristics (motility, carbol fuchsin stain and gram stain) and biochemical characteristics (acid or alkli production, utilization of different carbon sources, pH and NaCl tolerance, and starch and urea hydrolysis). We tested them on molecular level by amplification of 16s rRNA gene. All tested isolates came to be motile, gram-negative, not able to absorb Congo red. Results of biochemical testes showed that all tested isolates were acid producers, fast growers, able to survive on NaCl concentration ranging from 0.5 to 5%. All of them were able to grow in presence of wide range of pH (5 to 8). 16s rRNA gene came to be same size (about 1500 bp) in all Rhizobial isolates.