Analytical study of microsomes and isolated subcellular membranes from rat liver: I. Biochemical methods

Abstract

The series introduced by this paper reports the results of a detailed analysis of the microsomal fraction from rat liver by density gradient centrifugation. The biochemical methods used throughout this work for the determination of monoamine oxidase, NADH cytochrome c reductase, NADPH cytochrome c reductase, cytochrome oxidase, catalase, aminopyrine demethylase, cytochromes bs, and P 450, glucuronyltransferase, galactosyl-transferase, esterase, alkaline and acid phosphatases, 5'-nucleotidase, glucose 6-phos-phatase, alkaline phosphodiesterase I, N-acetyl-β-glucosaminidase, β-glucuronidase, nucleoside diphosphatase, aldolase, fumarase, glutamine synthetase, protein, phospholipid, cholesterol, and RNA are described and justified when necessary. © 1974, Rockefeller University Press., All rights reserved.