Drought regulation of GST8, encoding the Arabidopsis homologue of ParC/Nt107 glutathione transferase/peroxidase

Abstract

Glutathione transferases (GST; EC 2.5.1.18) have been involved in many biotic and ahiotic interactions of plants with their environment, hut very little information is available on their regulation and possible role in drought tolerance. The GST8 gene of Arabidopsis thaliana encodes, as assessed by phylogenetic analysis, the homologue of an extremely conserved subgroup of Tau GSTs. During rapid dehydration of seedlings and progressive drought stress of mature plants, GST8 transcript levels increased following slower kinetics than RNAs for dehydration responsive genes RD29A and B, and strictly paralleled, in a mostly ABA independent manner, expression of oxidative stress marker PRX. GST8 RNA levels were also consistently increased by oxidative stress, high doses of auxin or cytokinin, and to a lesser extent, by wounding. RNA levels of ERD13, a previously described rapid dehydration responsive GST of the Phi class, were not coregulated with those of GST8. Our results suggest that a drought-associated oxidative stress induces accumulation of GST8, whose function could he to counteract the effect of higher ROS production in stressed plants.