HPTLC pattern assessment of Avicennia marina stem and spectrometric analysis of the separated phyto-constituents

Abstract

The scientifi c evaluation, validation and authentication of medicinal plants are highly essential and key steps to achieve effective phytochemicals and drugs for disease prevention. The checklist of medicinal plants especially mangroves along with their scientifi c validation is still biased. The traditional methods are poor, time consuming as well as less scientifi c, so there is a need to used emerging technological knowledge and sophisticated analytical methods to fulfi ll this knowledge gap. Therefore, present study is an attempt to study the phytochemistry and recognize HPTLC pattern of Avicennia marinastem and spectrum analysis of resolved compounds in three different extracts. As a result, presence of different class of phytochemicals were detected. HPTLC determination densitometry analysis of 10 μl sample size under UV-258 nm of stem extract-1 (5 % NH 3) showed the presence of 2 components with Rf values ranging from 0.76-0.90; the stem extract-2 (methanol alone) revealed the presence of 4 components with Rf values in the range of 0.03-0.90; and the stem extract-3 (5 % HCl) revealed presence of 7 components with Rf values in the range of 0.03-0.90. As result, extract-3 (Slightly acidic) gave maximum number of separated compounds and characteristic fi ngerprinting pattern and may be useful as a biochemical integrity check marker for this plant as well as to assess the diversity and further characterization of phyto-compounds present in Avicennia marina stem.