Evaluation and utilization of a kinetic enzyme direct measuring photometer

Abstract

The author has recently utilized a prototype model of the Beckman Enzyme Activity Analyzer System TR in their laboratory measuring various serum enzyme activities which include: alkaline phosphatase (ALP), E.C.3.1.3.1; creatine kinase (CK), E.C.2.7.3.2; hydroxybutyrate dehydrogenase (HBD), E.C.1.1.1.30; lactate dehydrogenase (LD), E.C.1.1.1.27; aspartate transaminase (AST), E.C.2.6.1.1.; and alanine transaminase (ALT), E.C.2.6.1.2. Precision was found to be good. Sample activities could be measured as high as 1000 IU/l. The carryover studies fell within 2 SD of the means of the enzyme control studies. Coefficients of variation for ALP and CK were in the ranges of 0.40 to 2.14% and 0.52 to 4.30%, respectively. Correlation studies were done with GemSAEC and Gilford 300 N Spectrophotometer and the results were accurate, precise and reproducible.