Cytotoxicity and synergistic effect of biogenically synthesized ternary therapeutic nano conjugates comprising plant active principle, silver and anticancer drug on MDA-MB-453 breast cancer cell line

Abstract

Drug delivery through biogenically synthesized silver nanoparticles (AgNPs) in cancer treatment is exerted by smaller size entailing high surface area and synergistic effects of embedded biomolecules. In this study, prepared ternary conjugates of silver with plant active compound and anticancer drug towards reducing the dose through synergy, rendered by Electrostatic Attraction (EA) of functionalized drug on to the surface of biogenically synthesized AgNPs. The biogenic synthesis resulted in particles of nanometer range as well as serving reducing and capping agents. The cytotoxicity and synergistic effect of ternary therapeutic nano conjugates evaluated using MDA-MB-453 breast cancer cells were found to be superior than Doxorubicin (Dox). Quantitative HPTLC analysis showed 57.22 % inhibition by Dox-AP-AgNPs at a concentration of 2.5 μg/mL of Andrographolide and 0.95 μg/mL of Dox validating synergistic effect of the ternary conjugate.